Research Article
Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC
Table 1
Composition of growth media for culture of oMSC.
| 100 mL medium | F2 | F10 | F2-EGF | F10-EGF | F2-S | F10-S | F2-S-EGF | F10-S-EGF |
| DMEM 1 g/L glucose [mL]1 | 97 | 89 | 97 | 89 | 56.4 | 51.6 | 56.4 | 51.6 | L-Glutamine penicillin streptomycin (LGPS) [mL]1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| F | | | | | | | | | FCS [mL]2 | 2 | 10 | 2 | 10 | 2 | 10 | 2 | 10 |
| S | | | | | | | | | MCDB-201 [mL]3 | — | — | — | — | 37.6 | 34.4 | 37.6 | 34.4 | ITS + Premix 1x [mL]3 | — | — | — | — | 1 | 1 | 1 | 1 | Dexamethasone3, stock 100 mM [µL], final conc. 1 nM | — | — | — | — | 1 | 1 | 1 | 1 | L-Ascorbic acid3, stock 500 mM [mL], final conc. 100 µM | — | — | — | — | 2 | 2 | 2 | 2 |
| EGF | | | | | | | | | EGF [ng/mL]3 | — | — | 10 | 10 | — | — | 10 | 10 |
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PAA, Coelbe, Germany.
2Pan Biotech, Aidenbach, Germany.
3Sigma-Aldrich, Steinheim, Germany.
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