Research Article
Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC
Table 3
Adipogenic differentiation media according to standard protocols.
| Adipogenic differentiation | Protocol 1 [31] | Protocol 2 [21] |
| Adipogenic induction medium | | | DMEM 4.5 g/L glucose | | | FCS [%] | 10 | 10 | Dexamethasone [µM] | 1 | 1 | Indomethacin [µM] | 0.2 | 200 | Insulin [mg/mL] | 0.01 | 0.01 | 3-Isobutylxanthin [mM] | 0.05 | 0.5 | Cell density [/cm2] | 80.000 | 30.000 | Adipogenic maintenance medium | | | Serum [%] | 10 | | Insulin [mg/mL] | 0.01 | |
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