Research Article

Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC

Table 4

Chondrogenic differentiation media according to standard protocols.

Chondrogenic differentiationProtocol 1 [31]Protocol 2 [19]

DMEM 1 g/L glucose
 Serum depleted
Dexamethasone [µM]11
L-Ascorbic-acid-2-phosphate [mM] 0.170.17
Sodium pyruvate [µg/mL]100100
Proline [mM]0.050.05
ITS-Plus Premix [mL]55
Human transforming growth factor-ß3 (TGF-ß3)10 ng/mL
Human transforming growth factor-ß1 (TGF-ß1)10 ng/mL
Bone morphogenic protein-7 (BMP-7)100 ng/mL
Cell density [/pellet]1.000.00011.000.000

The original cell number of 250.000 hMSC per pellet [31] was adapted to 1.000.000 oMSC per pellet, as oMSC are considerably smaller than hMSC.