Research Article
Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC
Table 4
Chondrogenic differentiation media according to standard protocols.
| Chondrogenic differentiation | Protocol 1 [31] | Protocol 2 [19] |
| DMEM 1 g/L glucose | | | Serum depleted | | | Dexamethasone [µM] | 1 | 1 | L-Ascorbic-acid-2-phosphate [mM] | 0.17 | 0.17 | Sodium pyruvate [µg/mL] | 100 | 100 | Proline [mM] | 0.05 | 0.05 | ITS-Plus Premix [mL] | 5 | 5 | Human transforming growth factor-ß3 (TGF-ß3) | 10 ng/mL | | Human transforming growth factor-ß1 (TGF-ß1) | | 10 ng/mL | Bone morphogenic protein-7 (BMP-7) | | 100 ng/mL | Cell density [/pellet] | 1.000.0001 | 1.000.000 |
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The original cell number of 250.000 hMSC per pellet [31] was adapted to 1.000.000 oMSC per pellet, as oMSC are considerably smaller than hMSC.
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