Comparing the Gene Expression Profile of Stromal Cells from Human Cord Blood and Bone Marrow: Lack of the Typical “Bone” Signature in Cord Blood Cells
Figure 4
Lentiviral overexpression of OSX in two USSC cell lines. (a) Quantitative RT-PCR analysis of gene expression (in undifferentiated cells) after overexpression in relation to the control cells (Mock). RPL13A was used as housekeeping gene. (b) Osteogenic differentiation experiments in overexpressed and Mock cells. After 14 days of differentiation, Von Kossa and Alizarin Red S staining were performed in the induced (+) and non-induced (−) cells. Mineralized areas are stained in brown/black or red, respectively. Scale bar: 200 μm. The results were confirmed in independent experiments; representative histological staining and the subsequent quantification of the bound Alizarin Red S-dye (c) are depicted. ** to 0.005, very significant; ***, extremely significant (unpaired -test). OSX (SP7): osterix, RUNX2: runt-related transcription factor 2, BSP: integrin-binding sialoprotein, BMP2: bone morphogenetic protein 2, and RPL13A: ribosomal protein L13A.
