Dynamic Imaging of Marrow-Resident Granulocytes Interacting with Human Mesenchymal Stem Cells upon Systemic Lipopolysaccharide Challenge
Figure 1
Distribution of CMTPX-labeled hMSCs 24 hours after i.v. injection into a C57BL/6 mouse.FITC-dextran was injected 15–30 minutes before intravital TPM imaging to highlight the vasculature. The calvarium bone marrow (BM) was imaged immediately and all other tissues were removed and fixed overnight in 2% PFA for whole-organ TPM imaging the next day. (a) Representative high-resolution TPM images of lung (LU), liver (LV), lymph node (LN), spleen (SP), and bone marrow (BM) are shown. Blue: collagen (second-harmonics); Green: FITC-dextran vessel dye; Magenta: infused hMSCs. Outset scale bars = 50 μm; inset scale bars = 20 μm. (b) Numbers of CMTPX+ hMSC cells per mm3 of imaged tissue were enumerated.
