Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition

<table>Hepatic-specific protein expressions of the differentiated cells after induction for 18 days. Albumin (ALB) expression analysis: MSCDHCs generated from WJMSCs-SUT1 ((a)–(d)) and WJMSCs-SUT2 ((e)–(h)) were stained with rabbit polyclonal anti-human albumin antibody following the differentiation period. For detection of cytokeratin 18 (CK-18) expression, MSCDHCs generated from WJMSCs-SUT1 ((i)–(l)) and WJMSCs-SUT2 ((m)–(p)) were stained with mouse monoclonal anti-human cytokeratin 18 antibody following the differentiation period. Cells nuclei were counterstained with DAPI. Original magnifications: <svg height="11.125" id="M5" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 34.575001 11.125" width="34.575001" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> for all pictures. Bars indicate 50 <i>μ</i>m. </table>

Stem Cells International

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Figure 4

Figure 4: Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition 

Figure 4 | Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition 