Research Article

Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique

Figure 1

Effect of initial pelleting medium on MSC adipogenesis. Medium compositions were described in Section 2.2.3. (a): At one week (I) %, (II) %, (III) %, and (IV) % of the total cross-sectional area were Oil-red O positive (fraction of total aggregate area measured on at least 15 40x fields per condition per donor, ). (b): At 3 weeks overall: (I) %, (II) %, (III) %, and (IV) % of the total cross-sectional area were Oil-red O positive. (fraction of total aggregate area measured on at least 10 40x fields per condition per donor, ). Oil-Red O with hematoxylin counterstain, scale bars = 50 μm.
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(a)
806525.fig.001b
(b)