Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
Figure 6
CD133 immunoreactivity in capillary tubes. (a) In the later stages of culture (9-day culture), capillary tubes formed. Phase-contrast microscopy showed that many tubes were formed from the rat aortic explant. Arrows indicate CD133-positive tubes among CD133-negative tubes. Rat aortic explant (). (b) CD133-positive cells were present in a tubular pattern. Photograph showing CD133-positive tubes indicated by arrows in (a). Rat aortic explant (). Scale bar = 20 μm. (c) CD133 expression was clearly found in the tip region (arrow) of the tube rather than the stalk region near the rat aortic explant (). Scale bar = 20 μm.
