Dual Differentiation-Exogenous Mesenchymal Stem Cell Therapy for Traumatic Spinal Cord Injury Repair in a Murine Hemisection Model

Figure 1

A murine laminectomy and hemisection model of TSCI. Development of a murine laminectomy and hemisection model of TSCI was achieved using protocols approved by the University of Rochester Committee for Animal Resources (IACUC). After the animal is anesthetized, a laminectomy is performed to remove thorax 11 lamina (a), then the dura is opened to expose the spinal cord (b), and, finally, a hemisection lesion is performed to generate a 2 mm defect in the right half side of the spinal cord (c). Postoperatire dorsal view (d) and lateral view (e) of micro-CT scans of the spine; 5x (f) and 20x (g) micrographs of H&E stained histology sections are presented to illustrate the vertebral bone and spinal cord defects that generated in this model, respectively.