Improved Method for Ex Ovo-Cultivation of Developing Chicken Embryos for Human Stem Cell Xenografts
Immunohistochemical analysis of xenografted ITSCs. (a) Cryosections of embryonic chicken tissue harboring transplanted, human lacZ-positive cells. Xenografted integrated ITSCs revealed typical nestin expression (arrowheads). (b) Integrated ITSCs were positive for β-III-tubulin (arrowheads). (c) Integration of ITSCs in the basal layer of the epidermis was underlined by expression of CK14 (arrowheads) of xenografted cells. Transplantation experiments were performed as quadruplicate with consistent results. Representative results are shown.