|
| Gene | | Primer Sequence: 5′-3′ | Product (bp) | Annealing temperature |
|
| Angiopoetin-like 3 (ANGPTL-3) | NM_014495.2 | Forward:
TGGGAGGCTTGATGGAGAAT
Reverse:
GTAGCGTATAGTTGGTTTCG | 164 bp | 60°C |
| CCAAT enhancer binding protein alpha (CEBPα) | NM_004364.3 | Forward:
GAGTCACACCAGAAAGCTAG
Reverse:
GATGGACTGATCGTGCTTC | 184 bp | 60°C |
| Delta like-1 homologue (DLK-1) | NM_003836.5 | Forward:
AGCACCTATGGGGCTGAAT
Reverse:
GTCACGCACTGGTCACAAAG | 119 bp | 60°C |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | NM_002046.4 | Forward:
GAGTCAACGGATTTGGTCGT
Reverse:
TTGATTTTGGAGGGATCTCG | 238 bp | 60°C |
| Insulin-like growth factor 2 (IGF-2) | NM_000612.4,
NM_001007139.4,
NM_001127598.1 | Forward:
ATTGCTGCTTACCGCCCCA
Reverse:
CACTCCTCAACGATGCCACG | 143 bp | 60°C
|
| Insulin-like growth factor-binding protein 1 (IGFBP-1) | NM_000596.2 | Forward:
CAAAAAATGGAAGGAGCCC
Reverse:
GATGTCTCACACTGTCTGC | 153 bp | 60°C |
| Peroxisome proliferators activator gamma 2 (PPARγ2) | NM_015869.4 | Forward:
TCCATGCTGTTATGGGTGAA
Reverse:
TCAAAGGAGTGGGAGTGGTC | 193 bp | 60°C |
| Ribosomal protein L13a (RPL13a) | NM_001270491.1,
NM_012423.3 | Forward: GAGGTATGCTGCCCCACAAA
Reverse:
TTCAGACGCACGACCTTGAG | 136 bp | 60°C |
| Stem cell factor (SCF) | NM_003994.5,
NM_000899.4 | Forward:
GGATAAGCGAGATGGTAGTA
Reverse:
CTTCAGGAGTAAAGAGCC | 210 bp | 55°C
|
| Stromal-derived factor 1 (SDF-1) | NM_199168.3,
NM_000609.5,
NM_001033886.2,
NM_001178134.1 | Forward:
CTCAACACTCCAAACTGTGC
Reverse:
GCTTTCTCCAGGTACTCC | 110 bp | 60°C
|
| Thrombopoietin (THPO) | NM_000460.2,
NM_001177597.1,
NM_001177598.1 | Forward:
TCACCCTTTGCCTACACC
Reverse:
GTCACTGCTCCCAGAATGT | 108 bp | 60°C
|
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