Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Figure 1

Examples of chicken iPSC-like cells grown in different media conditions during 2nd passage growth. (a) Chicken iPSC-like cells grown in BRL-conditioned media. The cells became darker and detached from the bottom of the plate. They did not survive past the 2nd passage. (b) Cells grown in cESC media initially proliferated well but began to detach from the plate after the 2nd passage. They did not survive past the 4th passage. (c) Chicken iPSC-like cells grown in cESC and 2i+ media did not survive past the 4th passage. (d) Chicken iPSC-like cells grown in 2i+ media did not survive past the 6th passage. (e) Chicken iPSC-like cells grown in modified 2i+ media. The colony was much bigger than the colonies grown in the other 3 media conditions, and the scale bar is adjusted likewise.