Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Figure 3

qRT-PCR of chicken iPSC-like cells at the 7th passage and differentiated neurons in comparison to chicken embryonic fibroblasts. cNanog, cOct4, and cSox2 are chicken homologs of genes normally expressed in stem cells relative to fibroblast; chicken Sox2, DCX, Nestin, and beta-tubulin III are in neurons and GMFB in glial cells. The differentiated neuronal culture used was from 10 days after induction. Values below 0.4 are not shown in the figure (barely detectable). DCX and tubulin had no (zero) detectable expression in chicken iPSC-like cells cultured in 2i+ modified media. cNanog and cOct4 had no detectable expression in differentiated neurons. Glia maturation factor had 0.72 ± 0.21 relative expressions and Nestin had 0.0047 ± 0.00034 in PRPSCs. The values are from independent replicates. Error bars, SEM. , relative to fibroblast controls.