Research Article

Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Figure 5

Differentiation of chicken iPSC-like cells (ciPSC), grown in modified 2i+ media, into neurons. (a) Undifferentiated chicken iPSC-like cells in modified 2i+ media at passage 10. (b) Embryoid bodies in suspension culture in embryoid body media, 2 days after initiation of differentiation protocol. (c) Attached embryoid body in transition media, 6 days after initiation of differentiation. (d) Neurite outgrowth (arrow) in N2B27 media at day 9 since the start of differentiation. (e) Developed neurons (arrows) in N2B27 media at day 15.