Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Figure 8

Whole-cell current-clamp recordings of differentiated chicken neurons generated from iPSC-like cells reveal action potential firing. (a) DIC image showing a single cell (arrow) with recording pipette in whole-cell configuration (arrowhead). (b) Membrane potential responses generated by current injection. Suprathreshold depolarizing currents resulted in trains of overshooting action potentials. (c) Expanded view of a single action potential illustrating narrow spike width and strength after hyperpolarization.

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