Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Table 1

Ingredients of the five passaging media conditions presented in this study.

Media name	BRL-C. plus	cESC	2i+	cESC 2i+	2i+ mod.
KO DMEM	82%	87%	82%	87%	82%
FBS	15%	10%	15%	10%	15%
Na Pyruvate (100 mM)	1 mM	1 mM	1 mM	1 mM	1 mM
Glutamax (100x)	2 mM	1 mM	1 mM	1 mM	1 mM
Nonessential amino acids (100x)	1 mM	1 mM	1 mM	1 mM	1 mM
Beta-mercaptoethanol	0.005 mM	0.005 mM	0.005 mM	0.005 mM	0.005 mM
Penicillin/Streptomycin (100x)	1 mM	1 mM	1 mM	1 mM	1 mM
Nucleoside (100x)	1 mM	—	—	—	—
LIF	—	1000 U/mL	1000 U/mL	1000 U/mL	50 U/mL
PD0325901	—	—	0.5 μM	0.5 μM	1 μM
CHIR99021	—	—	3 μM	3 μM	3 μM
A83-01	—	—	0.5 μM	0.5 μM	1 μM
rhIL6	—	1 ng/mL	—	1 ng/mL	—
rhsILR6a	—	1 ng/mL	—	1 ng/mL	—
rmSCF	—	1 ng/mL	—	1 ng/mL	—
rhIGF1	—	1 ng/mL	—	1 ng/mL	—
rhFGF2	—	1 ng/mL	—	1 ng/mL	—

The media are all similar in a set of standard cell culture media ingredients (from KO DMEM to Penicillin/Streptomycin), but differ in the presence and concentrations of some of those ingredients, as well as LIF, 2i+ inhibitors (PDO325901, CHIR99021, and A83-01), and recombinant human or mouse cytokine factors (rh or rm) that inhibit cell differentiation.