Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs
Figure 4
Comparison between optimized miR/PEI/MNP complexes and commercially available magnetic transfection reagents. Freshly isolated hMSCs were transfected with Cy3-labeled miR/PEI/MNP complexes composed of an N/P ratio of 10 combined with 5 pmol miR bound to 1 μg/mL MNPs ((a), (b)) or an N/P ratio of 2.5 combined with 10 pmol miR bound to 1 μg/mL MNPs ((c), (d)). For comparison with commercially available magnetic transfection complexes, appropriate amounts of Lipofectamine 2000 and CombiMag particles were used. 24 hours after transfection, uptake efficiency ((a), (c)) and cytotoxicity ((b), (d)) of complexes were determined by flow cytometry, while a magnetic field was applied. Untransfected cells were used as control. Values are presented as mean ± SEM; .
