Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model

Table 1

Primers used for real-time PCR amplification.

Gene	GenBank accession number	Forward primer (5′-3′); Reverse primer (5′-3′)	PCR product size (bp)
eNOS	NM_021838-2	TGGAAATTAACGTGGCTGTG; GCCTTCTGCTCATTTTCCAA	112
ET-1	M64711.1	TGTCTACTTCTGCCACCTGGA; CCTAGTCCATACGGGACGAC	69
TNF-α	BC107671.1	GTCTACTGAACTTCGGGGTGA; ATGAGAGGGAGCCCATTTG	67
MMP-9	NM_031055.1	AAAAGGCATCCAGCATCTGT; AGCTGTCGGCTGTGGTTC	88
α-MHC	NM_017239.2	CGAAACTGAAAACGGCAAG; TGGCCATGTCCTCGATCT	92
β-MHC	NM_017240.1	GCTGCAGAAGAAGCTCAAAGA; GCAGCTTCTCCACCTTGG	98
Bax	NM_017059.1	GTGAGCGGCTGCTTGTCT; GACTCCAGCCACAAAGATGG	107
Caspase 3	NM_012922.2	AAACCTCCGTGGATTCAAAA; AGCCCATTTCAGGGTAATCC	123
Bcl-2	NM_016993.1	GGGATGCCTTTGTGGAACT; CTGAGCAGCGTCTTCAGAGA	82
α-actin	U19893-1	CTGGGGCCTGAGGAGTTC; CCGGTTGAACTCAGCATCA	87

eNOS = endothelial nitric oxide synthase; ET-1 = endothelin-1; TNF-α = tumor necrotic factor alpha; MMP = matrix metalloproteinase; MHC = myosin heavy chain; PCR = polymerase chain reaction.