Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model

Table 2

Baseline characteristics of three group animals at day 28 after hypoxia-induced pulmonary arterial hypertension.

Variable	Group 1 ( = 10)	Group 2 ( = 10)	Group 3 ( = 10)	-value^*
Body weight (g)	24.91 ± 1.39	25.04 ± 2.32	25.08 ± 1.36	0.977
RBC count (1.0 × 10^6/mL)	8.14 ± 0.33^a	10.10 ± 1.05^b	10.12 ± 1.52^b	<0.001
Hemoglobulin (gm/dL)	11.83 ± 0.45^a	14.73 ± 1.52^b	14.61 ± 2.27^b	<0.001
RVSP (mm Hg)	20.89 ± 1.69^a	39.31 ± 3.37^b	29.57 ± 1.77^c	<0.001
LVSP (mm Hg)	103.38 ± 10.48	94.96 ± 15.11	94.97 ± 15.07	0.410
RV weight (g)	0.021 ± 0.012^a	0.028 ± 0.003^b	0.023 ± 0.025^a	<0.001
Total HW (g)	0.107 ± 0.404	0.127 ± 0.015	0.119 ± 0.125	0.119
Ratio of RV/total HW	0.178 ± 0.015^a	0.222 ± 0.302^b	0.189 ± 0.018^a	<0.001
Total lung weight (g)	0.145 ± 0.009^a	0.193 ± 0.027^b	0.160 ± 0.028^a	<0.001

Data are expressed as means ± SD.
RBC = red blood cell; RVSP = right ventricular systolic pressure; LVSBP = left ventricular systolic blood pressure; HW = heart weight.
Group 1 = sham control; group 2 = hypoxia + negative control siRNA; group 3 = hypoxia + siRNA-TRPC1.
^*By one-way ANOVA.
Different letters (a, b, c) are being used for grouping, showing significant difference (at level 0.05) among different groups by Tukey’s multiple comparison procedure.