Research Article

Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos

Table 1

Sequences of primers specific for microsatellite markers.

LocusPrimer sequence (5→3)Conditions
DenaturationAnnealingNumber of cyclesExtension

D2Mit296CAACTGTAAATCCAGTCGTAGGG
CTCTGCTGAGGTTACTGTGGG
94°C, 30 sec55°C, 30 sec405 min

D3Mit51GGCACTGATAGCAGGCCTAG
TCTCTTCTGGTATTTCCTTCCG
94°C, 60 sec55°C, 60 sec2510 min

D11Mit20CCTGTCCAGGTTTGAGAGGA
CTTGGGAGCCTCTTCGGT
94°C, 60 sec55°C, 60 sec3510 min

D13Mit88ACTGATGGCTCATGAGACCC
AAAATTAATAGGAACTGCAAGGG
94°C, 30 sec55°C, 30 sec405 min

D16Mit139GTATGTAAGGAATGGTCAAATTCTTG
TCATTGTGATTGTGAAAGAATGC
94°C, 30 sec55°C, 30 sec405 min

D19Mit10GCCTTTAAGCCAGTCAAGACA
CCAGTCTGGACTTGTGAATGA
94°C, 3 sec55°C, 30 sec405 min