Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue

Figure 3

Characterization of eMSCs and BMSCs. (a) Growth curves of eMSCs and BMSCs. (b) Representative histograms of the cell surface marker expression on eMSCs (p6) and BMSCs (p3) analysed by flow cytometry, the labelled cells represented by the green line populations (M1), and relevant isotype-matched cells depicted by the blue line (M2). (c) Immunofluorescent staining for mesoderm and stemness markers. Antibody staining (green or red) with DAPI nuclear staining (blue) (bars = 50 μm). (d) Transmission electron microscopy of eMSCs (p6). (A), (B): eMSCs bars = 0.5 μm, “N”: nucleus of cell; “M”: mitochondrion; “F”: filaments fibres; “G”: Golgi apparatus; “R”: rough endoplasmic reticulum.

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