Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue

Figure 7

(a) Homogeneous cell encapsulation and high viability of embedded cells in the Pluronic F-127. (b) Oil Red O staining showed lipids in Pluronic F-127. (c) Subcutaneous injection of adipogenically induced eMSCs-hydrogels implants in nude mice after 4 weeks. (d) Gross view of the newly formed adipose tissue. (e), (f) H&E ((e)(A), (e)(B)) and Masson’s trichrome ((f)(A), (f)(B)) staining of adipose tissue sections showed that mature adipocytes and functional blood vessels are observed in the specimen, bars = 100 μm. (g) The CM-Dil labeled cells in tissue sections from the newly formed tissue. Bars = 100 μm. (h) Transmission electron microscopy shows the implants (A) and extracellular matrix (B), “N”: nucleus of cell; “L”: lipid droplet, bars = 2 μm, 200 nm.

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