The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells
Figure 6
Variation of neuronal markers seven days after incubation with BMSCs and HUCPVCs CM. Levels of mRNA for different neuronal markers were quantified by quantitative real-time RT-PCR and normalized to both undifferentiated/proliferative cells (reference level: 1) and HBMS housekeeping gene. Quantification of neuronal markers expression revealed that BMSCs CM 24 h and 96 h displayed a significant increase in the SH-SY5Y cells expression of DRD2 gene when compared to RA-differentiated cells (). However, BMSCs CM 96 h simultaneously induced a decrease in synaptophysin in comparison with RA-differentiated cells (). On the other hand, for all the neuronal markers studied, no statistically significant differences were found between HUCPVCs CM 24 h and RA groups (). Yet, HUCPVCs CM 96 h significantly elevated mRNA levels of DRD2 and DAT genes when compared with both BMSCs 96 h (; ) and RA-differentiated cells (; ). The later results suggest that HUCPVCs CM 96 h is inducing SH-SY5Y cells towards the DAergic phenotype. In addition, differences between BMSCs and HUCPVCs from the same time point indicate that different tissue derived MSCs secretome have distinct effects in SH-SY5Y cells differentiation with respect to neuronal phenotype. For all the other neuronal markers studied, no significant statistical differences were observed between all CM tested conditions and RA-differentiated cells (), which strongly indicates that the CM from BMSCs and HUCPVCs are capable of inducing SH-SY5Y cells neuronal differentiation. Moreover, the different expression pattern of neuronal markers exhibited by SH-SY5Y cells among CM time points of collection indicates that the effects mediated by MSCs secretome in SH-SY5Y cells differentiation is related with the temporal profile of CM collection (values are shown as mean ± SEM, ). Symbols correspondence to statistical signification: (1) refers to comparisons between RA-differentiated cells and MSCs CM and (2) # regards the correlation between MSCs CM from the same time point (, ).
