Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells

Figure 2

Lineage differentiation of untagged (a, c, and e) and DiD-labeled MSCs (b, d, and f) into adipocytes, osteocytes, and NPs. Culturing of unlabeled and DiD-labeled MSCs in differentiation media followed by staining with Oil Red O for lipid (a and b, resp.; original magnifications: 400x) or Alizarin Red for calcium deposition (c and d, resp.; original magnifications: 400x) and microscopic examinations for NPs production (e and f, resp.) were used to confirm their similar differentiation capacity (original magnifications: 100x).

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