Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro
Figure 3
FACS analysis. (A) (a) ASCs at the second passage were separated into four populations: CD90(−)/CD105(−) (green), CD90(+)/CD105(−) (light blue), CD90(−)/CD105(+) (dark blue), and CD90(+)/CD105(+) (brown). ASCs pink in color were not categorized with any group. ((b)–(e)) ASCs were cultured in DMEM without osteogenic supplements (normal), ((f)–(i)) ASCs were cultured in osteogenic medium (AdenoX-bmp2(−)), whereas ((j)–(m)) ASCs were also transfected with AdenoX-bmp2 (AdenoX-bmp2(+)). (B) Ratios of the populations of the sorted ASCs altered in the three different culture media after 14-day culture, which resulted in changes of percentages of cells positive for CD90 or CD105: CD90(+)/CD105(−) plus CD90(+)/CD105(+) as CD90-positive, and CD90(−)/CD105(+) plus CD90(+)/CD105(+) as CD105-positive. The results are expressed as means of three replicates using three different batches of ASCs. Statistical significance was identified as follows: ⊖, decreased compared to that in normal medium of 14-day culture; ⊕, increased compared to that in normal medium of 14-day culture ().
