Migration, Proliferation, and Differentiation of Cord Blood Mesenchymal Stromal Cells Treated with Histone Deacetylase Inhibitor Valproic Acid
Figure 3
VPA increases MMP-2 but not MT1-MMP expression. CB-derived MSC were incubated with or without (control) 1, 2.5, 5, and 10 mM VPA for 3 h or 6 h. (a) The expression of MMP-2 mRNA (top panel) was evaluated by real-time quantitative RT-PCR (qRT-PCR) using 18S mRNA as internal calibrator. The secretion of pro-MMP-2 and active MMP-2 was examined using zymography (bottom panel). The numbers at the bottom of the gel correspond to the fold-increase in pro-MMP-2 expression relative to control. (b) The expression of MT1-MMP mRNA as determined by qRT-PCR was not affected by treatment of the cells with VPA.
