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Stem Cells International
Volume 2014, Article ID 610495, 14 pages
Research Article

Migration, Proliferation, and Differentiation of Cord Blood Mesenchymal Stromal Cells Treated with Histone Deacetylase Inhibitor Valproic Acid

1Centre for Innovation (Formerly Research & Development), Canadian Blood Services, 8249-112 Street, Edmonton, AB, Canada T6G 2R8
2Division of Hematology, Department of Medicine, University of Alberta, 8440-112 Street, Edmonton, AB, Canada T6G 2B7

Received 25 June 2013; Revised 30 January 2014; Accepted 4 February 2014; Published 16 March 2014

Academic Editor: Pavla Jendelova

Copyright © 2014 Leah A. Marquez-Curtis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1(A): Heterogeneity of adherent layer formed by plating cord blood (CB)-derived mononuclear cells (upper panel). At passage 4, a more homogeneous population of cells with fibroblastoid morphology is obtained (lower panel).

upplementary Figure 1(B): Immunophenotypic characterization of CB MSC. Cells showed negative expression for the hematopoietic markers CD45 and CD34 and positive expression for the stromal markers CD90, CD105 and CD73. The black lines represent isotypic controls while the colored lines and shaded area represent antigen of interest.

  1. Supplementary Material