Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5
Figure 5
Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μM KT5823 and 1 μM T0156 (a, d), 1 μM sildenafil (b, e), or 10 μM zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni’s post-test), *, ** and ***, significantly different from control and + and ++ significantly different from the PDE5 inhibitor.