Differentiation of Equine Mesenchymal Stromal Cells into Cells of Neural Lineage: Potential for Clinical Applications

Figure 11

Expression of nestin in undifferentiated and differentiated high passaged eBM-MSCs. Confocal microscopy shows the expression of nestin (red) in neural crest-like cells (left panel) and in undifferentiated controls (right panel) from eBM-MSCs of middle-aged horse after 12 h of chemical induction. DAPI was used to stain the nuclei (blue). Note that nestin is inconsistently found perinuclearly or diffusely in the cytoplasm in the differentiated cells. Expression of nestin in the control is perinuclear. Additionally, based on the intensity of the signal, its expression appears less when compared to cells from the low passage. Scale bar = 25 μm.