Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation
Kinetics of implantation of hMSCs during 60 days after abdominal irradiation. The percentage of positive mice for the human β-globin gene detected by PCR (AI) has been calculated after abdominal irradiation (AI). The human gene has been detected in (a) stomach, (b) jejunum, (c) lungs, (d) liver, (e) kidney, (f) spleen, and (g) bone marrow. The tissues were collected at 3, 15, 30, and 60 days after acute AI. The percentages represent the ratio (number of positive mice for the human beta-globin presence divided by total number of animals tested). At each analysis time, each group consisted of 10 animals. The control (named sham) consists in nonirradiated mice injected with hMSCs. The dotted line represents the median of the positive animal percentage during the 60 days after radiation exposure. (h) Illustration of anatomical variations in the presence of MSC after acute abdominal radiation.