Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation

Table 1

hMSC engraftment in nonirradiated mice 3 days after injection.

Organs	Number of positive; mice for 10 mice
Brain	10/10 (100%)
Heart	5/10 (50%)
Lung	10/10 (100%)
Liver	10/10 (100%)
Spleen	10/10 (100%)
Abdominal area
Kidney	10/10 (100%)
Muscle	0/10 (0%)
Stomach	9/10 (90%)
Duodenum	2/10 (20%)
Jejunum	10/10 (100%)
Ileum	1/10 (10%)
Cecum	1/10 (10%)
Proximal colon	1/10 (10%)
Distal colon	1/10 (10%)
Posterior leg
Skin	1/10 (10%)
Muscle	4/10 (40%)
Bone	3/10 (30%)
Bone marrow	10/10 (100%)
Blood	3/10 (30%)
Number of positive organs	102/190
Frequency	54%

Human DNA accounted for engraftment. hMSCs implanted in mouse tissues were detected by PCR of the gene for human 𝛽-globin. The rate of implantation of human cells was determined on a series of non-irradiated mice grafted with hMSC injection at 5·10⁶ cells per mouse. The percentages represent the ratio of positive mice for the presence of human 𝛽-globin on the total number of mice analyzed.