Stem Cells International / 2014 / Article / Tab 1

Research Article

Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation

Table 1

hMSC engraftment in nonirradiated mice 3 days after injection.

OrgansNumber of positive
mice for 10 mice

Brain10/10 (100%)
Heart5/10 (50%)
Lung10/10 (100%)
Liver10/10 (100%)
Spleen10/10 (100%)
Abdominal area
 Kidney10/10 (100%)
 Muscle0/10 (0%)
 Stomach9/10 (90%)
 Duodenum2/10 (20%)
 Jejunum10/10 (100%)
 Ileum1/10 (10%)
 Cecum1/10 (10%)
 Proximal colon1/10 (10%)
 Distal colon1/10 (10%)
Posterior leg
 Skin1/10 (10%)
 Muscle 4/10 (40%)
 Bone 3/10 (30%)
 Bone marrow10/10 (100%)
Blood3/10 (30%)
Number of positive organs102/190
Frequency54%

Human DNA accounted for engraftment. hMSCs implanted in mouse tissues were detected by PCR of the gene for human -globin. The rate of implantation of human cells was determined on a series of non-irradiated mice grafted with hMSC injection at 5·106 cells per mouse. The percentages represent the ratio of positive mice for the presence of human -globin on the total number of mice analyzed.