Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

Figure 5

Phenotypic and functional characterization of SVF isolated by the manual and automated process. (a) Detection of pericytes in SVF isolated by the manual and automated process. The CD31−ve cells in the SVF were gated (depicted in blue) and analyzed for expression of CD34 and CD146. Values represent percentage of CD31−ve cells expressing CD34 or CD146. Data shows a representative set of plots from four individual donors in each group. (b) Semiquantitative RT–PCR analysis for expression of angiogenic markers in SVF obtained from manual and automated process. 18s ribosomal RNA expression was used to normalize cDNA concentration for each sample set. Data depicts gene expression in two pairs of samples out of four pairs of individual donors analysed. Man: manual; Auto: automated. (c) Clonal expansion potential of SVF obtained by manual and automated process. Data represents mean number of CFU-F obtained for each plating density from four individual donors, each assayed in duplicate. Errors represent SD from four individual donors.

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