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Stem Cells International
Volume 2015, Article ID 109353, 11 pages
Research Article

Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

Stempeutics Research Pvt. Ltd., Akshay Tech Park Nos. 72 and 73, 2nd Floor, EPIP Zone, Phase 1, Whitefield, Bangalore 560066, India

Received 30 September 2014; Revised 27 February 2015; Accepted 9 April 2015

Academic Editor: Christian Dani

Copyright © 2015 Swathi SundarRaj et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1: Verification of the accuracy of manual counting of SVF was performed by direct comparison of the manual counting method against counts obtained with an automated image based cell counter. Difference between the two methods is not significant (p=0.19, paired t-test).

Supplementary Table 2: Effect of peristaltic pump was studied to ensure that the pump action does not cause mechanical damage to the tissue and cells. The yield and viability of SVF isolated from the lipoaspirate tissue before and after passing through the peristaltic pump was found to be comparable (Supplementary table 2).

Supplementary Figure 1: Retention of cells on the 5 μm filter was visualized by staining with Toluidene blue. Representative images for the stained filters is provided in Supplementary figure 1.

  1. Supplementary Material