Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age
Live ASC isolation relative to duration of cryopreservation. (a) ASCs were isolated from 32 patients whose adipose tissue was cryopreserved for varying amounts of time (range 2–1159 days, average cells/g tissue). Live ASCs isolated ranged from 0 to cells/g adipose, average cells/g tissue, showing a trend toward decreased live ASC isolation with increasing ASC cryopreservation duration. (b) Live cell count was compared relative to cryopreservation duration in 3 cohort groups: <1 year (), 1-2 years (), and >2 years (). A significant decrease in live ASC isolation was observed between the >2 years and <1 year cryopreservation duration cohorts; . (c) Patient age was compared for each of the cryopreservation cohort groups and no significant differences were found between any group; .
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