Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age

Figure 1

Live ASC isolation relative to duration of cryopreservation. (a) ASCs were isolated from 32 patients whose adipose tissue was cryopreserved for varying amounts of time (range 2–1159 days, average cells/g tissue). Live ASCs isolated ranged from 0 to cells/g adipose, average cells/g tissue, showing a trend toward decreased live ASC isolation with increasing ASC cryopreservation duration. (b) Live cell count was compared relative to cryopreservation duration in 3 cohort groups: <1 year (), 1-2 years (), and >2 years (). A significant decrease in live ASC isolation was observed between the >2 years and <1 year cryopreservation duration cohorts; . (c) Patient age was compared for each of the cryopreservation cohort groups and no significant differences were found between any group; .

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