Research Article
Acute Lymphoblastic Leukemia Cells Inhibit the Differentiation of Bone Mesenchymal Stem Cells into Osteoblasts In Vitro by Activating Notch Signaling
Table 2
Primers sequences for real-time RT-PCR analysis.
| | Gene | Length | Annealing temperature (°C) | Sequence |
| | OCN | 81 | 60.7 | GGTGCAGCCTTTGTGTCCA | | GGCTCCCAGCCATTGATACA |
| | OPN | 81 | 63 | GGCCGAGGTGATAGTGTGGTT | | AGCATCAGGGTACTGGATGTCA |
| | Hes1 | 313 | 63.5 | AAAATGCCAGCTGATATAATGGAG | | GGTCTGTGCTCAGCGCAGCCGTCA |
| | Notch1 | 76 | 63.5 | CGGGTCCACCAGTTTGAATG | | GTTGTATTGGTTCGGCACCAT |
| | Runx2 | 101 | 62.3 | TTATTCTGCTGAGCTCCGGAA | | AACTCTTGCCTCGTCCACTCC |
| | Jagged1 | 164 | 60 | GCTGCCTTTCAGTTTCGC | | CGCCCGTGTTCTGCTTCA |
| | GADPH | 114 | 54 | CCACATCGCTCAGACACCAT | | GGCAACAATATCCACTTTACCAGA |
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