Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones

Table 1

Primers used for RT-PCR.

Gene	Primer	Annealing (°C)	Cycle	Expressed cells
FLK	F: 5′-AAGGCTCAAACCAGACAAGC-3′; R: 5′-TTCCTGCTGGTGGAAAGAAC-3′	58	30	Early mesoderm
PDGFRα	F: 5′-GGGGAGAGTGAAGTGAGCTG-3′; R: 5′-AGTCTCGGGATCAGTTGTGC-3′	58	30
FOXA2	F: 5′-CCGGATCGAGGACAAGTGAG-3′; R: 5′-CAACAACAGCAATGGAGGAG-3′	58	30	Endoderm
SOX17	F: 5′-AAGGGCGAGTCCCGTATCC-3′; R: 5′-TTGTAGTTGGGGTGGTCCTG-3′	58	30
TTF-1	F: 5′-ACCAGGACACCATGAGGAAC-3′; R: 5′-GCGCCGACAGGTACTTCTG-3′	58	30, 40	Lung progenitor
p63	F: 5′-TTGCCCCTCCTAGTCATTTG-3′; R: 5′-TACTGTCCGAAACTTGCTGC-3′	58	33	Basal
FOXJ1	F: 5′-TGGATCACGGACAACTTCTG-3′; R: 5′-AAGTTGCCTTTGAGGGGTTC-3′	58	40	Ciliated
MUC5AC	F: 5′-TACGTGTTCTCCGAGCACTG-3′; R: 5′-GGTTCCACATGAGGACAAGG-3′	58	30	Goblet
CC10	F: 5′-GTCACACTGGCTCTCTGCTG-3′; R: 5′-GAGCAGTTGGGGATCTTCAG-3′	58	40	Clara
AQP5	F: 5′-ATCTTCGCCTCCACTGACTC-3′; R: 5′-TTTCTTCTTTTCCCCCTTGG-3′	56	40	Alveolar type I
T1α	F: 5′-TGGGGTCTTACTAGCCATCG-3′; R: 5′-TAGAGGAGCCAAGTCTGGTG-3′	58	40
CFTR	F: 5′-GCCAGCGTTGTCTCCAAAC-3′; R: 5′-CGATAGAGCGTTCCTCCTTG-3′	58	30	Alveolar type II
SPB	F: 5′-AAGTTCCTGGAGCAGGAGTG-3′; R: 5′-AGAGGAATGGGGAATTGCTG-3′	58	40
SPC1	F: 5′-TTGGTCCTTCACCTCTGTCC-3′; R: 5′-CTCCCACAATCACCACGAC-3′	58	40
SPC2	F: 5′-AACGCCTTCTTATCGTGGTG-3′; R: 5′-AAGACTGGGGATGCTCTCTG-3′	58	35
CD133	F: 5′-GCAGGGATTATTCTATGCTGTG-3′; R: 5′-ACGCCTTGTCCTTGGTAGTG-3′	58	35	Cancer stem
HIF-1α	F: 5′-TGCTCATCAGTTGCCACTTC-3′; R: 5′-TCTCATTTCCTCATGGTCAC-3′	56	30	Cancer
18SrRNA	F: 5′-TACCTGGTTGATCCTGCCAGTAGGAT-3′; R: 5′-CCCGTCGGCATGTATTAGCTCTAGAA-3′	58	17