| A549 clones | Characteristics of cloned cells |
| Group number | Plate A | Plate B | Days to confluent | Cell thickness | Cell morphology | Boundary of cell cluster | Cell density | Clusterius | Others |
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(1) | E2, E5, H9, H10 | A2, A3, B2, C6, F5, F9, G1, G2, H10, H11 | 3.5 | Thin | Spindle | Unclear | Low | Low | | (2) | | C10, C11 | 4 | Thin | Polygonal | Clear | High | High | | (3) | C5, E8 | A8, D12, G5 | 3.5 | Thin | Polygonal | Clear | Low | High | | (4) | G8 | | 14 | Thick | Polygonal | Unclear | Low | High | Piling up, granule-rich | (5) | | A10, E12 | 3.5 | Thin | Polygonal | Unclear | Low | Low | | (6) | B7, F5 | C2, C4, C12, G8 | 3.5 | Thick | Spindle | Unclear | Low | Low | | (7) | F8, H12 | | 4.5 | Thick | Polygonal | Clear | High | High | | (8) | | B6, B7, B10, C1 | 4 | Thick | Polygonal | Unclear | High | Low | | (9) | A12 | | 3.5 | Thick | Polygonal | Unclear | Low | Low | | (10) | | D2 | 3 | Thick | Polygonal | Unclear | Low | Low | Granule-rich | (11) | H11 | A5, B3, C8, D7, G7 | 3.5 | Thick | Polygonal | Unclear | Low | Low | | (12) | G9-1, G9-2 | | 4.5 | Thick | Invadopodia | Unclear | Low | Low | Bright cell body |
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Optical observations of isolated 46 clones were summarized and categorized into 12 groups. Cell thickness, mean gray value ≥5: thick, <5: thin. Boundary of cell cluster: smoothly draw the outline of cluster: clear; hard to draw the outline: unclear. Cell density, the number of cells in the frame of 100 μm × 100 μm ≥10: high, <10: low. Clustering: the number of cell cluster ≥3: high, <3: low.
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