Table 3: Optical analyses of A549 clones.

A549 clonesCharacteristics of cloned cells

Group numberPlate APlate BDays to confluentCell thicknessCell morphologyBoundary of cell clusterCell densityClusteriusOthers

(1)E2, E5, H9, H10A2, A3, B2, C6, F5,
F9, G1, G2, H10, H11
3.5ThinSpindleUnclearLowLow
(2)C10, C114ThinPolygonalClearHighHigh
(3)C5, E8A8, D12, G53.5ThinPolygonalClearLowHigh
(4)G814ThickPolygonalUnclearLowHighPiling up, granule-rich
(5)A10, E123.5ThinPolygonalUnclearLowLow
(6)B7, F5C2, C4, C12, G83.5ThickSpindleUnclearLowLow
(7)F8, H124.5ThickPolygonalClearHighHigh
(8)B6, B7, B10, C14ThickPolygonalUnclearHighLow
(9)A123.5ThickPolygonalUnclearLowLow
(10)D23ThickPolygonalUnclearLowLowGranule-rich
(11)H11A5, B3, C8, D7, G73.5ThickPolygonalUnclearLowLow
(12)G9-1, G9-24.5ThickInvadopodiaUnclearLowLowBright cell body

Optical observations of isolated 46 clones were summarized and categorized into 12 groups. Cell thickness, mean gray value ≥5: thick, <5: thin. Boundary of cell cluster: smoothly draw the outline of cluster: clear; hard to draw the outline: unclear. Cell density, the number of cells in the frame of 100 μm × 100 μm ≥10: high, <10: low. Clustering: the number of cell cluster ≥3: high, <3: low.