Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction

Figure 1

Expressions of EGFP and hBcl-xL in rat bone marrow MSCs. (a) Schematic representation of the coding regions of the viral vectors pLenti6.3-IRES-EGFP and pLenti6.3-hBcl-xL-IRES-EGFP. In vector pLenti6.3-hBcl-xL-IRES-EGFP, the insertion of IRES could lead to the individual expression of EGFP together with the expression of hBcl-xL in the transduced cells. All expressions were driven by CMV promoter. ((b)–(g)) Expressions of EGFP in wild type MSCs ((b) and (c)), vector-MSCs ((d) and (e)), and hBcl-xL-MSCs ((f) and (g)). (h) Western blot analysis of the expressions of hBcl-xL in MSCs, vector-MSCs, and hBcl-xL-MSCs. Data are representative of three independent experiments.

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