Expression profiles of pluripotency genes in single if-iPSC colonies from NGFP, 4F2A, and mESC groups. (a) Representative gene expression density profiles of single if-iPSC colonies in the mESC, NGFP if-iPSC, and 4F2A if-iPSC groups. Each panel represents a density profile of a single colony (4 profiles per each group) and the corresponding gel image is shown left with lanes of individual gene groups (Figure 1
(e)). AxioVision intensity profile tool (v4.8) was used for peak profiles. (b) Gene expression profiles (GEPs) of individual colonies. Spline-type plots are shown where individual lines in different colors denote the expression profiles of the pluripotency genes in individual colonies. (c) Mean GEPs of individual iPSC colony groups. The Gapdh
level is set as the normalization standard. Slc38a5
is included as a control that was previously shown to be more expressed in if-iPSCs than in stabilized iPSC and ESCs [23
]. Error bars, standard error.