Research Article

HLA-E: Presentation of a Broader Peptide Repertoire Impacts the Cellular Immune Response—Implications on HSCT Outcome

Table 1

Canonical and noncanonical HLA-E peptide ligands.

Peptide sequenceSourceReference

Canonical HLA-E peptide ligands (reported in literature)
Peptide position
123456789
VMAPRTLILHLA-Cw signal peptide[50]
VMAPRTLFLHLA-G signal peptide[24, 28]
VMAPRTLLLHLA-01 signal peptide[51]
VMAPRTLVLHLA-02 signal peptide[51]
ALALVRMLIATP binding cassette transporter[52]
QMRPVSRVLHeat shock protein 60[30]
AISPRTLNAHIV gag protein[22]
SQQPYLQLQGliadin-wheat protein[23]
SQAPLPCVLEBV-BZLF1 protein[24]

Noncanonical HLA-01:01 peptide ligands
12345678910111213141516
TLQASNQSEIIQKQVVIsoform 2 of nucleosome-remodeling factor subunit BPTF
ILDLSSMAPQALVQFWPOLQ DNA polymerase theta
TRIFGDALASIKQQAQIsoform 1 of serine/arginine repetitive matrix protein
RMGLAKVSLSPVITEMPLEC1 Isoform 6 of Plectin-1
GGIVTLSQAAGDVDARIsoform 6 of Nesprin-1
IKDDEKEAEEGEDDRDHNRPC protein
VPRLGSTFSLDTSMSCBL E3 ubiquitin-protein ligase CBL
HLIGIHFTGCSMNPAAQP6 Aquaporin-6
VVTGNMGSNDKVGDFDSG1 Desmoglein-1
LVDSGAQVSVVHPNLASPRV1 retroviral-like aspartic protease 1
LGHPDTLNQGEFKELCalprotectin S100A9
GRCIQMWFDSAQGNFOLR3 folate receptor 3 precursor
MSFLWPVHAEPNPDPutative uncharacterized protein TTC39A
APIVGGEMAVLALLCOL29A1 Isoform 1 of Collagen alpha-5(VI) chain
GQGSNGQGSSSHSSIRS4 Insulin receptor substrate 4
LTPFPGPGPRRPPWLMTK3 lemur tyrosine kinase 3
YRSGGGFSSGSAGIKRT1 Keratin, type II cytoskeletal 1
SLINNHIPCLISGPutative uncharacterized protein HMGN1
PKKTESHHKAKGKHistone H2A type 3
DVHDGKVVSTHEQKRT14 Keratin, type I cytoskeletal 14
ASGYVSSADLVFTIsoform 2 of Cytokine receptor common subunit beta
LDGKVISFEGCAVOlfactory receptor 10S1
VSEKGTVQQADE7 kDa protein
PRVMTPPSDEPDProtocadherin Fat 1
AVTFSSLPAAITcDNA FLJ59480, highly similar to Smoothelin
GPQRCGWPDGLGAP2 associated kinase 1
PSLTSVTTTFVOlfactory receptor 51D1
SKGKIYPVGY52 kDa protein
PGHCEDVLVLIsoform 2 of Urea transporter 2
APPPPEPALHomo sapiens selective LIM binding factor
QPHPGDQSEHSPA1L putative uncharacterized protein
ILNFPPPPIsoform 2 of Caprin-2
IVESRPVPXRN2 Isoform 1 of 5′-3′ exoribonuclease 2
IIAIFGPGIsoform 1 of multidrug resistance protein 3
MKAAPGVESolute carrier family 12, member 6 isoform c
AAVLEYLHistone H2A type 2-B

Canonical HLA-E peptide ligands are reported to induce HLA-E surface expression, and noncanonical HLA-E peptide ligands are peptides that were identified within this study. The peptides selected for stabilization and cytotoxicity studies are highlighted in bold.