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Stem Cells International
Volume 2015, Article ID 369828, 11 pages
http://dx.doi.org/10.1155/2015/369828
Research Article

High OCT4 and Low Expressions Determine In Vitro Lifespan of Mesenchymal Stem Cells

1Hospital Israelita Albert Einstein, 627/701, 05652-900 São Paulo, SP, Brazil
2Orthopedic Division, Federal University of Sao Paulo, 783, 04038-031 São Paulo, SP, Brazil

Received 8 April 2015; Accepted 6 May 2015

Academic Editor: Jia Xie

Copyright © 2015 Carla A. Piccinato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W. Wagner, P. Horn, M. Castoldi et al., “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS ONE, vol. 3, no. 5, article e2213, 2008. View at Google Scholar
  2. J. Campisi and F. d'Adda di Fagagna, “Cellular senescence: when bad things happen to good cells,” Nature Reviews Molecular Cell Biology, vol. 8, no. 9, pp. 729–740, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. L. Hayflick and P. S. Moorhead, “The serial cultivation of human diploid cell strains,” Experimental Cell Research, vol. 25, no. 3, pp. 585–621, 1961. View at Publisher · View at Google Scholar · View at Scopus
  4. J.-H. Chen, C. N. Hales, and S. E. Ozanne, “DNA damage, cellular senescence and organismal ageing: causal or correlative?” Nucleic Acids Research, vol. 35, no. 22, pp. 7417–7428, 2007. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Campisi, “Cell biology: the beginning of the end,” Nature, vol. 505, no. 7481, pp. 35–36, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. F. Rodier and J. Campisi, “Four faces of cellular senescence,” Journal of Cell Biology, vol. 192, no. 4, pp. 547–556, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. G. P. Dimri, X. Lee, G. Basile et al., “A biomarker that identifies senescent human cells in culture and in aging skin in vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 20, pp. 9363–9367, 1995. View at Publisher · View at Google Scholar · View at Scopus
  8. B. Y. Lee, J. A. Han, J. S. Im et al., “Senescence-associated beta-galactosidase is lysosomal beta-galactosidase,” Aging Cell, vol. 5, no. 2, pp. 187–195, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. C. J. Sherr and F. McCormick, “The RB and p53 pathways in cancer,” Cancer Cell, vol. 2, no. 2, pp. 103–112, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. J. Gil and G. Peters, “Regulation of the INK4b-ARF-INK4a tumour suppressor locus: all for one or one for all,” Nature Reviews Molecular Cell Biology, vol. 7, no. 9, pp. 667–677, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Campisi and F. D. di Fagagna, “Cellular senescence: when bad things happen to good cells,” Nature Reviews Molecular Cell Biology, vol. 8, no. 9, pp. 729–740, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. T. Tchkonia, Y. Zhu, J. van Deursen, J. Campisi, and J. L. Kirkland, “Cellular senescence and the senescent secretory phenotype: therapeutic opportunities,” Journal of Clinical Investigation, vol. 123, no. 3, pp. 966–972, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. M. C. Velarde, J. M. Flynn, N. U. Day, S. Melov, and J. Campisi, “Mitochondrial oxidative stress caused by Sod2 deficiency promotes cellular senescence and aging phenotypes in the skin,” Aging, vol. 4, no. 1, pp. 3–12, 2012. View at Google Scholar · View at Scopus
  14. R. Iglesias-Bartolome, V. Patel, A. Cotrim et al., “MTOR inhibition prevents epithelial stem cell senescence and protects from radiation-induced mucositis,” Cell Stem Cell, vol. 11, no. 3, pp. 401–414, 2012. View at Publisher · View at Google Scholar · View at Scopus
  15. S. C. Johnson, P. S. Rabinovitch, and M. Kaeberlein, “MTOR is a key modulator of ageing and age-related disease,” Nature, vol. 493, no. 7432, pp. 338–345, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. B. Gharibi, S. Farzadi, M. Ghuman, and F. J. Hughes, “Inhibition of Akt/mTOR attenuates age-related changes in mesenchymal stem cells,” Stem Cells, vol. 32, no. 8, pp. 2256–2266, 2014. View at Publisher · View at Google Scholar
  17. B. G. Childs, D. J. Baker, J. L. Kirkland, J. Campisi, and J. M. van Deursen, “Senescence and apoptosis: dueling or complementary cell fates?” EMBO Reports, vol. 15, no. 11, pp. 1139–1153, 2014. View at Publisher · View at Google Scholar
  18. J. Nichols, B. Zevnik, K. Anastassiadis et al., “Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4,” Cell, vol. 95, no. 3, pp. 379–391, 1998. View at Publisher · View at Google Scholar · View at Scopus
  19. C.-C. Tsai, P.-F. Su, Y.-F. Huang, T.-L. Yew, and S.-C. Hung, “Oct4 and Nanog directly regulate Dnmt1 to maintain self-renewal and undifferentiated state in mesenchymal stem cells,” Molecular Cell, vol. 47, no. 2, pp. 169–182, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. W. Wagner, S. Bork, P. Horn et al., “Aging and replicative senescence have related effects on human stem and progenitor cells,” PLoS ONE, vol. 4, no. 6, Article ID e5846, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. K. J. Livak and T. D. Schmittgen, “Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method,” Methods, vol. 25, no. 4, pp. 402–408, 2001. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. M. J. Whitfield, W. C. J. Lee, and K. J. van Vliet, “Onset of heterogeneity in culture-expanded bone marrow stromal cells,” Stem Cell Research, vol. 11, no. 3, pp. 1365–1377, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. W. Wagner, A. D. Ho, and M. Zenke, “Different facets of aging in human mesenchymal stem cells,” Tissue Engineering Part B: Reviews, vol. 16, no. 4, pp. 445–453, 2010. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Ren, D. F. Stroncek, Y. Zhao et al., “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: Molecular changes associated with BMSC senescence,” Stem Cell Research, vol. 11, no. 3, pp. 1060–1073, 2013. View at Publisher · View at Google Scholar · View at Scopus
  26. D. C. Colter, R. Class, C. M. DiGirolamo, and D. J. Prockop, “Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 7, pp. 3213–3218, 2000. View at Publisher · View at Google Scholar · View at Scopus
  27. D. Zipori, “The hemopoietic stem cell niche versus the microenvironment of the multiple myeloma-tumor initiating cell,” Cancer Microenvironment, vol. 3, no. 1, pp. 15–28, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. A. Muraglia, R. Cancedda, and R. Quarto, “Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model,” Journal of Cell Science, vol. 113, no. 7, pp. 1161–1166, 2000. View at Google Scholar · View at Scopus
  29. A. Schellenberg, S. Mauen, C. M. Koch, R. Jans, P. De Waele, and W. Wagner, “Proof of principle: quality control of therapeutic cell preparations using senescence-associated DNA-methylation changes,” BMC Research Notes, vol. 7, no. 1, article 254, 2014. View at Publisher · View at Google Scholar · View at Scopus
  30. K. R. Shibata, T. Aoyama, Y. Shima et al., “Expression of the p16INK4A gene is associated closely with senescence of human mesenchymal stem cells and is potentially silenced by DNA methylation during in vitro expansion,” Stem Cells, vol. 25, no. 9, pp. 2371–2382, 2007. View at Publisher · View at Google Scholar · View at Scopus
  31. Z. Gu, X. Cao, J. Jiang et al., “Upregulation of p16INK4A promotes cellular senescence of bone marrow-derived mesenchymal stem cells from systemic lupus erythematosus patients,” Cellular Signalling, vol. 24, no. 12, pp. 2307–2314, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. J. W. Shay and I. B. Roninson, “Hallmarks of senescence in carcinogenesis and cancer therapy,” Oncogene, vol. 23, no. 16, pp. 2919–2933, 2004. View at Publisher · View at Google Scholar · View at Scopus
  33. J.-P. Coppé, C. K. Patil, F. Rodier et al., “Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor,” PLoS Biology, vol. 6, no. 12, pp. 2853–2868, 2008. View at Google Scholar · View at Scopus
  34. R. Salama, M. Sadaie, M. Hoare, and M. Narita, “Cellular senescence and its effector programs,” Genes & Development, vol. 28, no. 2, pp. 99–114, 2014. View at Publisher · View at Google Scholar · View at Scopus
  35. J. Wu, J. Niu, X. Li, X. Wang, Z. Guo, and F. Zhang, “TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production,” BMC Developmental Biology, vol. 14, no. 1, article 21, 2014. View at Publisher · View at Google Scholar · View at Scopus
  36. Z. Wang, E. Oron, B. Nelson, S. Razis, and N. Ivanova, “Distinct lineage specification roles for NANOG, OCT4, and SOX2 in human embryonic stem cells,” Cell Stem Cell, vol. 10, no. 4, pp. 440–454, 2012. View at Publisher · View at Google Scholar · View at Scopus
  37. S. Geissler, M. Textor, J. Kühnisch et al., “Functional comparison of chronological and in vitro aging: differential role of the cytoskeleton and mitochondria in mesenchymal stromal cells,” PLoS ONE, vol. 7, no. 12, Article ID e52700, 2012. View at Publisher · View at Google Scholar · View at Scopus