Physical Activity Increases the Total Number of Bone-Marrow-Derived Mesenchymal Stem Cells, Enhances Their Osteogenic Potential, and Inhibits Their Adipogenic Properties

Figure 1

Analysis of MSC (a) phenotypes using flow cytometry. The FACS profile of separated Sca-1 positive and Lin-negative cells demonstrating , , and MSCs (a). (b) Morphology of MSCs cultured in DMEM medium supplemented with 10% of FBS (standard culture) and the results of its chondrogenic, osteogenic, and adipogenic stimulation. For visualization of glycosaminoglycans and calcium deposits specific Safranin O and Alizarin Red staining were used, respectively, while lipid droplets were stained using Oil Red O.

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