Decrease in neural differentiation of precursor cells by an Axin stabilizer (IWR-1-endo) from days 4 to 6 in N2B27 medium. (a)–(f) GFP expression of 46C cells was elevated by BIO treatment (0.75 μM) from days 4 to 6, whereas it was reduced by IWR-1-endo (0.75 μM) treatment from days 4 to 6. Cells were cultured in N2B27 medium for 6 days. (g) FACS analysis of Sox1-GFP expression during monolayer differentiation in N2B27 medium. (h) Proportions of Sox1-GFP expressing cells as determined by FACS. Scale bars, 100 μm.