Morphometric Analysis of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes: Determining the Maturation State of a Population by Quantifying Parameters in Individual Cells

Figure 2

Effect of isoproterenol and oleic acid on the area of hESC-vCMs. ((a) panel (A), (a) panel (B)) Representative examples of the typical areas of hESC-vCMs measured. These two cells (labeled with an α-actinin antibody) have cell areas of ((a) panel (A)) 2,170 μm² and ((a) panel (B)) 10,025 μm², respectively. Scale bar is 30 μm. (b–g) Histograms to show the distribution of cells (in %) exhibiting a particular cell area in (b) untreated controls and (c–g) following treatment with (c) 0.3 μM isoproterenol, (d) 100 μM oleic acid, (e) 200 μM oleic acid, (f) 0.3 μM isoproterenol plus 100 μM oleic acid, or (g) 0.3 μM isoproterenol plus 200 μM oleic acid. The number of cells analyzed is indicated in the upper left corner of each graph. In all treatment groups, none of the area values was significantly different from those of the untreated controls. The arrows indicate the mean cell area determined for the population of cells.

(a)

(b) Untreated control

(c) 0.3 μM isoproterenol

(d) 100 μM oleic acid

(e) 200 μM oleic acid

(f) 0.3 μM isoproterenol + 100 μM oleic acid

(g) 0.3 μM isoproterenol + 200 μM oleic acid