Characterization of Human Knee and Chin Adipose-Derived Stromal Cells

Figure 5

UCP1 expression in adipocytes generated from chin ASCs. ASCs derived from chin (C) and knee (K) were induced to undergo differentiation for 10 days. (a) RNAs were prepared and UCP1 expression was determined by real-time PCR. paired depots. Corresponding data points of knee and chin paired depots are connected with lines. (b) Cells were stimulated (+) or not (−) with forskolin for 4 h and then proteins were prepared. Expression of UCP1 was assessed by Western-blotting. Tubulin was used as loading control.

(a)

(b)