Neuroprotective and Antiapoptotic Activity of Lineage-Negative Bone Marrow Cells after Intravitreal Injection in a Mouse Model of Acute Retinal Injury

Figure 3

Longitudinal follow-up of the fate of Lin⁻BMCs at different time points post intravitreal transplantation. Immunofluorescence analysis of retinas after intravitreal GFP⁺Lin⁻BMCs delivery showed transplanted cells (green) on the 7th day (a, b) and at 3 months after injection (c). Quantitative analysis of the total number of incorporated Lin⁻BMCs per eye section (/time point) (d). Coreactivity of GFP (green) and Isolectin-B4 (red) by fluorescent lectin staining showed that transplanted GFP⁺Lin⁻BMCs locally differentiated into Isolectin-B4-positive macrophages (inserts) in Lin⁻BMC-injected eyes on the 7th day after transplantation (e) and at 3 months after injection (f). Scale bar = 20 μm. for day 7 versus other time points.

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