Neural Progenitor Cells Derived from Human Embryonic Stem Cells as an Origin of Dopaminergic Neurons
Low CBF1 response of neural progenitor cells. ((a)–(c)) CBF1 response of cells was measured by transient transfection of GFP-reporter plasmid. ((a) and (b)) Phase contrast and fluorescence images of neural progenitor cells and neural progenitor/stem cells, respectively. Scale bar represented 50 μM for all images. (c) Histogram showed percentage of CBF1-responsive cells. (d) Histogram presented luciferase activity controlled by CBF1. Three independent experiments were performed with a standard deviation bar and represented (Student’s t-test).
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