Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Figure 1

TIAR silencing E14 cells maintained their self-renewal pluripotency. (a) Quantitative RT-PCR analysis of the gene expression of TIAR in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. (b) Western blot analysis of the expression of TIAR in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. (c) RT-PCR analysis of the expression of Oct4, Nanog, Sox2, and Klf4 in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. E14 cells and mouse embryonic fibroblasts (MEFs) were used as a control. (d) Immunofluorescence analysis of the expression of Oct4 in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells (200x). by one-way ANOVA. Control: pSIREN-RetroQ control E14 cells; TIAR RNAi: TIAR RNAi E14 cells.

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