Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Figure 4

Expression of exogenous RNAi-resistant TIAR rescues the function of TIAR in siRNA-treated cells. (a) Western blot analysis of the expression of TIAR and c-myc in control, TIAR RNAi, expressional control, and expressional TIAR. (b) Quantitative RT-PCR analysis of the gene expression of nestin, Pax6, Flk-1, brachyury, Sox17, and Gata6 on day 4 during EB differentiation in TIAR RNAi, expressional control, and expressional TIAR. (c) Western blot analysis of the gene expression of nestin, Flk-1, and Sox17 on day 4 during EB differentiation in expressional control and expressional TIAR. by one-way ANOVA. Control: E14 cells infected with an empty control pCDNA3.1-Hygro vector; TIAR RNAi: TIAR RNAi E14 cells; expressional control: TIAR RNAi E14 cells infected with an empty control pCDNA3.1-Hygro vector; expressional TIAR: TIAR RNAi E14 cells infected with pCDNA3.1-Hygro vector containing RNAi-resistant TIAR transgene.

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